• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: None
  Constituents: PBS

• Concentration 50 µg at 1 mg/ml

• PurityProtein G purified
• Purification notesImmunoglobulin (IgG2A) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS and then lyophilized. Purity was analysed using electrophoresis, 4-12% Bis Tris Gel (Invitrogen).
• Clonality Monoclonal
• Clone numberMLR2
• IsotypeIgG2a
• Research Areas
  • Neuroscience
  • Neurology process
  • Growth and Development
  • Neurotrophins
  • Stem Cells
  • Neural Stem Cells
  • Surface Molecules
  • Stem Cells
  • Mesenchymal Stem Cells
  • Surface Molecules

Applications

Our Abpromise guarantee covers the use of ab62122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

• Application notesIs unsuitable for ,BL or WB.

Target

• FunctionLow affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
• Sequence similaritiesContains 1 death domain.
  Contains 4 TNFR-Cys repeats.
• DomainDeath domain is responsible for interaction with RANBP9.
  The extracellular domain is responsible for interaction with NTRK1.
• Post-translational
  modificationsN- and O-glycosylated.
  O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
  Phosphorylated on serine residues.
• Cellular localizationMembrane.
• Information by UniProt

• Database links

  • Entrez Gene: 4804 Human
  • Entrez Gene: 18053 Mouse
  • Entrez Gene: 24596 Rat
  • Omim: 162010 Human
  • SwissProt: P08138 Human
  • SwissProt: Q9Z0W1 Mouse
  • SwissProt: P07174 Rat
  • Unigene: 415768 Human

• Alternative names

  • CD271 antibody
  • CD271 antigen antibody
  • Gp80 LNGFR antibody

Anti-p75 NGF Receptor antibody [MLR2] (FITC) images

• 

  Immunocytochemistry/ Immunofluorescence - p75 NGF Receptor antibody [MLR2] (FITC) (ab62122)

  Ab62122 (1-2 ug/ml) intracellular staining of p75NTR in NSC34 motor neuron cell line by live confocal imaging.

• 

  Flow Cytometry - p75 NGF Receptor antibody [MLR2] (FITC) (ab62122)

  Overlay histogram showing PC12 cells stained with ab62122 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62122, 1/10 dilution) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x10⁶ cells) with secondary antibody DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with methanol (5 min) used under the same conditions.

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Abcamab12098Rabbit polyclonal to Arp8100ug3996Arp8hystemcell一抗

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Abcamab12112Rabbit polyclonal to STAT4100ul3996STAT4hystemcell一抗

Abcamab12113Rabbit polyclonal to STAT5a50ul3996STAT5ahystemcell一抗

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Abcamab1212Mouse monoclonal [OX42] to CD11b/c equivalent （FITC）500ul3996CD11b/chystemcell一抗

Abcamab12120Mouse monoclonal [44C143] to TLR850ug3996TLR8hystemcell一抗

Abcamab12121Mouse monoclonal [26C593] to TLR950ug3996TLR9hystemcell一抗

Abcamab12122Mouse monoclonal [33A1293] to TRAF250ug3996TRAF2hystemcell一抗

Abcamab12123Mouse monoclonal [55A219] to TRAF550ug3996TRAF5hystemcell一抗

Abcamab12124Mouse monoclonal [55B709.3] to TRAIL50ug3996TRAILhystemcell一抗

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Abcamab12142Mouse monoclonal [72B587] to IKKi/IKKe50ug3996IKK iota/IKK epsilonhystemcell一抗

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